Measurement of in vivo proliferation in human colorectal mucosa using bromodeoxyuridine - 20 Downloads
Measurement of in vivo proliferation in human colorectal mucosa using bromodeoxyuridine
C S Potten, M Kellett, S A Roberts, D A Rew, G D Wilson
Abstract
In vivo bromodeoxyuridine (BrdUrd) labelling of the human large bowel was performed and a detailed histochemical localisation of label in' sections of crypts was undertaken using a monoclonal antibody to BrdUrd containing DNA. Flow cytometric studies on extracted nuclei were also performed (data presented elsewhere). The average crypt in the human large bowel (excluding the rectum) was 82 cells in height and 41 cells in circumference, with a total of about 2000 cells (assuming a topographical correction factor of 0.6).
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